Osthole enhances the bone mass of senile osteoporosis and stimulates the expression of osteoprotegerin by activating β-catenin signaling

Abstract Introduction Osthole has a potential therapeutic application for anti-osteoporosis. The present study verified whether osthole downregulates osteoclastogenesis via targeting OPG. Methods In vivo, 12-month-old male mice were utilized to evaluate the effect of on bone mass. vitro, marrow stem cells (BMSCs) isolated and extracted from 3-month-old OPG −/− littermates +/+ mice. Calvaria osteoblasts 3-day-old C57BL/6J or Results significantly increased gene protein levels in primary BMSCs dose-dependent manner. deletion did not affect β-catenin expression. inhibited expression BMSCs, indicating that stimulates activation signaling. Conclusion attenuates osteoclast formation by stimulating β-catenin-OPG signaling could be drug senile osteoporosis.